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atcc 6303 strain  (ATCC)


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    Structured Review

    ATCC atcc 6303 strain
    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
    Atcc 6303 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+6303+strain/Streptococcus+Pneumoniae/bio_rxiv__64898__2026__01__10__698720-139-7-7
    Average 96 stars, based on 580 article reviews
    atcc 6303 strain - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Early metabolic reprogramming licenses Streptococcus pneumoniae for Influenza-driven superinfection"

    Article Title: Early metabolic reprogramming licenses Streptococcus pneumoniae for Influenza-driven superinfection

    Journal: bioRxiv

    doi: 10.64898/2026.01.10.698720

    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae ATCC-6303. Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
    Figure Legend Snippet: a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae ATCC-6303. Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .

    Techniques Used: Infection, Virus, Two Tailed Test

    Related Articles

    Mouse Assay:

    Article Title: Pertussis Toxin Improves Immune Responses to a Combined Pneumococcal Antigen and Leads to Enhanced Protection against Streptococcus pneumoniae
    Article Snippet: .. Repeated nasal immunizations with PspA5-wP induce high levels of anti-PspA5 IgG and IgA in the serum and in the respiratory mucosa of mice ( 19 ), but the depletion of complement in mice immunized with up to 6 doses of PspA5-wP completely abrogated protection against respiratory invasive challenge with the ATCC 6303 strain. ..

    Suspension:

    Article Title: Design, development, and assessment of a novel multi-peptide vaccine targeting PspC, PsaA, and PhtD proteins of Streptococcus pneumoniae.
    Article Snippet: Pneumococcus is the top cause of diseases such as pneumonia/meningitis, and of secondary infections after viral respiratory diseases like COVID-19/flu.. Pneumococcal protein-based vaccines consisting of proteins with various functions in virulence might provide a qualified alternative for present vaccines.. In this project, PspC, PsaA, and PhtD proteins were considered to anticipate B/T-cell epitopes using immunoinformatics to develop 4 multipeptide constructs (C, A, and D individual constructs, and a fusion construct CAD).

    Activity Assay:

    Article Title: Design, development, and assessment of a novel multi-peptide vaccine targeting PspC, PsaA, and PhtD proteins of Streptococcus pneumoniae.
    Article Snippet: Pneumococcus is the top cause of diseases such as pneumonia/meningitis, and of secondary infections after viral respiratory diseases like COVID-19/flu.. Pneumococcal protein-based vaccines consisting of proteins with various functions in virulence might provide a qualified alternative for present vaccines.. In this project, PspC, PsaA, and PhtD proteins were considered to anticipate B/T-cell epitopes using immunoinformatics to develop 4 multipeptide constructs (C, A, and D individual constructs, and a fusion construct CAD).

    Produced:

    Article Title: Design, development, and assessment of a novel multi-peptide vaccine targeting PspC, PsaA, and PhtD proteins of Streptococcus pneumoniae.
    Article Snippet: Pneumococcus is the top cause of diseases such as pneumonia/meningitis, and of secondary infections after viral respiratory diseases like COVID-19/flu.. Pneumococcal protein-based vaccines consisting of proteins with various functions in virulence might provide a qualified alternative for present vaccines.. In this project, PspC, PsaA, and PhtD proteins were considered to anticipate B/T-cell epitopes using immunoinformatics to develop 4 multipeptide constructs (C, A, and D individual constructs, and a fusion construct CAD).

    Bacteria:

    Article Title: Design, development, and assessment of a novel multi-peptide vaccine targeting PspC, PsaA, and PhtD proteins of Streptococcus pneumoniae.
    Article Snippet: Pneumococcus is the top cause of diseases such as pneumonia/meningitis, and of secondary infections after viral respiratory diseases like COVID-19/flu.. Pneumococcal protein-based vaccines consisting of proteins with various functions in virulence might provide a qualified alternative for present vaccines.. In this project, PspC, PsaA, and PhtD proteins were considered to anticipate B/T-cell epitopes using immunoinformatics to develop 4 multipeptide constructs (C, A, and D individual constructs, and a fusion construct CAD).

    In Vivo:

    Article Title: Design, development, and assessment of a novel multi-peptide vaccine targeting PspC, PsaA, and PhtD proteins of Streptococcus pneumoniae.
    Article Snippet: Pneumococcus is the top cause of diseases such as pneumonia/meningitis, and of secondary infections after viral respiratory diseases like COVID-19/flu.. Pneumococcal protein-based vaccines consisting of proteins with various functions in virulence might provide a qualified alternative for present vaccines.. In this project, PspC, PsaA, and PhtD proteins were considered to anticipate B/T-cell epitopes using immunoinformatics to develop 4 multipeptide constructs (C, A, and D individual constructs, and a fusion construct CAD).

    other:

    Article Title: Early metabolic reprogramming licenses Streptococcus pneumoniae for Influenza-driven superinfection
    Article Snippet: Together, these data suggest that in the ATCC-6303 strain, carbon source availability exerts a dominant influence over adh induction, with hypoxia playing a more limited modulatory role compared to observed in strain D39L.

    Injection:

    Article Title: Design, development, and assessment of a novel multi-peptide vaccine targeting PspC, PsaA, and PhtD proteins of Streptococcus pneumoniae.
    Article Snippet: Pneumococcus is the top cause of diseases such as pneumonia/meningitis, and of secondary infections after viral respiratory diseases like COVID-19/flu.. Pneumococcal protein-based vaccines consisting of proteins with various functions in virulence might provide a qualified alternative for present vaccines.. In this project, PspC, PsaA, and PhtD proteins were considered to anticipate B/T-cell epitopes using immunoinformatics to develop 4 multipeptide constructs (C, A, and D individual constructs, and a fusion construct CAD).

    Cell Culture:

    Article Title: Early metabolic reprogramming licenses Streptococcus pneumoniae for Influenza-driven superinfection
    Article Snippet: Strain-specific transcriptional responses were also observed; for example, genes involved in arginine biosynthesis ( argG and argH ) were selectively upregulated in ATCC-6303 (Extended Data Fig. 10 d, e ). .. Based on the conservation of adhE/A and malP/G/X upregulation in the ATCC-6303 strain during superinfection, we next assessed the importance of alternative carbon sources in transcriptional reprogramming, and cultured the bacterium in the presence of various mono-, di-, tri- and poly-saccharides. ..

    Incubation:

    Article Title: Pertussis Toxin Improves Immune Responses to a Combined Pneumococcal Antigen and Leads to Enhanced Protection against Streptococcus pneumoniae
    Article Snippet: .. Sera were collected 21 days after the last immunization, and pools (from 6 mice) were incubated with the ATCC 6303 strain. ..



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    ATCC atcc 6303 strain
    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
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    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
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    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
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    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
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    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
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    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae <t>ATCC-6303.</t> Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .
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    Image Search Results


    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae ATCC-6303. Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .

    Journal: bioRxiv

    Article Title: Early metabolic reprogramming licenses Streptococcus pneumoniae for Influenza-driven superinfection

    doi: 10.64898/2026.01.10.698720

    Figure Lengend Snippet: a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae ATCC-6303. Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .

    Article Snippet: Together, these data suggest that in the ATCC-6303 strain, carbon source availability exerts a dominant influence over adh induction, with hypoxia playing a more limited modulatory role compared to observed in strain D39L.

    Techniques: Infection, Virus, Two Tailed Test

    a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae ATCC-6303. Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .

    Journal: bioRxiv

    Article Title: Early metabolic reprogramming licenses Streptococcus pneumoniae for Influenza-driven superinfection

    doi: 10.64898/2026.01.10.698720

    Figure Lengend Snippet: a , Schematic of the mouse infection model. Mice were intranasally (i.n.) primed with PBS or influenza A virus (IAV; 10 PFU) and one week later infected with S. pneumoniae ATCC-6303. Bacterial inocula were ∼1 × 10⁸ CFU (D39L) or ∼1 × 10⁶ CFU (ATCC-6303) for pneumococcal infection alone (Spn) and ∼1 × 10⁴ CFU (D39L) or ∼1 × 10³ CFU (ATCC-6303) for superinfected (SI) mice. Fomepizole (10 mg kg⁻¹ in 200 ul of PBS) or PBS were administered intraperitoneally at the time of bacterial infection and again 12 h post infection. Mice were euthanized 24 h after bacterial challenge. b,c , Lung (b) and spleen (c) bacterial burdens in mice infected with S. pneumoniae D39L. d,e , Lung (d) and spleen (e) bacterial burdens in mice infected with S. pneumoniae ATCC-6303. Data are pooled from at least two independent experiments. Dots indicate individual mice (lungs n = 12-26, spleens n = 6-12), bars represent geometric means with geometric s.d. (b-e) . Statistical significance was determined by two-tailed unpaired t -tests (b-e) .

    Article Snippet: To assess whether our findings on the adaptation of pneumococcus to the IAV-primed lung extend beyond the D39L strain (serotype 2), we performed parallel experiments using the strain ATCC-6303, a serotype 3 isolate.

    Techniques: Infection, Virus, Two Tailed Test